cercopithecus aethiops bsc 40 kidney epithelial cells female Search Results


african green monkey kidney epithelial vero cells  (ATCC)
99
ATCC african green monkey kidney epithelial vero cells
African Green Monkey Kidney Epithelial Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transient transfection monkey kidney epithelial cos 7 cells  (ATCC)
99
ATCC transient transfection monkey kidney epithelial cos 7 cells
Transient Transfection Monkey Kidney Epithelial Cos 7 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monkey epithelial kidney cells vero e6  (ATCC)
99
ATCC monkey epithelial kidney cells vero e6
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Monkey Epithelial Kidney Cells Vero E6, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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african green monkey kidney  (ATCC)
96
ATCC african green monkey kidney
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
African Green Monkey Kidney, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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african green monkey kidney epithelial bs c 1 cells  (ATCC)
96
ATCC african green monkey kidney epithelial bs c 1 cells
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
African Green Monkey Kidney Epithelial Bs C 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cv 1 monkey kidney epithelial cells  (ATCC)
99
ATCC cv 1 monkey kidney epithelial cells
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Cv 1 Monkey Kidney Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bsc 40  (ATCC)
96
ATCC bsc 40
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Bsc 40, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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grivet (chlorocebus aethiops) kidney epithelial vero e6 cells  (BEI Resources)
90
BEI Resources grivet (chlorocebus aethiops) kidney epithelial vero e6 cells
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Grivet (Chlorocebus Aethiops) Kidney Epithelial Vero E6 Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vero (cercopithecus aethiops kidney epithelial normal) cell line  (National Centre for Cell Science)
90
National Centre for Cell Science vero (cercopithecus aethiops kidney epithelial normal) cell line
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Vero (Cercopithecus Aethiops Kidney Epithelial Normal) Cell Line, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptides treatment cos 1 monkey kidney epithelial  (ATCC)
98
ATCC peptides treatment cos 1 monkey kidney epithelial
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Peptides Treatment Cos 1 Monkey Kidney Epithelial, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc crl 1658 vero cells  (ATCC)
99
ATCC atcc crl 1658 vero cells
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
Atcc Crl 1658 Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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african green monkey  (ATCC)
99
ATCC african green monkey
A Inhibition of classical secretory pathway in YFV infected C6/36 and <t>Vero</t> <t>E6</t> cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).
African Green Monkey, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A Inhibition of classical secretory pathway in YFV infected C6/36 and Vero E6 cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).

Journal: bioRxiv

Article Title: The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex

doi: 10.1101/370932

Figure Lengend Snippet: A Inhibition of classical secretory pathway in YFV infected C6/36 and Vero E6 cells at 72hpi. B Inhibition of classical secretory pathway in ZIKV infected C6/36 and Vero E6 cells at 48hpi. C ZIKV NS1 secretion is reduced in C6/36 cells treated with CsA 9 μM. D Co-localization between YFV NS1 or ZIKV NS1 and CAV-1 in mosquito and VeroE6 cells. C6/36, Aag2 and VeroE6 cells were infected at MOI of 1 for 48 h. Cells were probed for CAV-1 (shown in red), viral NS1 (shown in green) and nuclei stained with DAPI (blue). E Pearson correlation coefficients (PCC) for CAV1-NS1 were measured in at least 20 confocal independent images with 0.47 μM laser sections. The bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences are denoted by *(P ≤0.05). F Protein sequence alignment of caveolin binding domain (CBD) in Flavivirus NS1. Symbols: Φ, aromatic amino acid (F/Y/W) and X, any amino acid. A , B and C ; Cells were infected with YFV or ZIKV at MOI of 1 for 1 h. The cells were washed 3 times and after treated with DMSO (control), 7μM BFA, 27μM GCA or 9μM CsA. For ZIKV infection supernatants were harvested at 48 hpi and for YFV infection supernatants were collected 72 hpi. Secreted ZIKV NS1 was measured using Platelia NS1 Ag (Bio-Rad) and represented as percentage of secreted NS1 compared to DMSO. Secreted YFV NS1 was evaluated by western blot. The experiments were performed in triplicate, and the bars represent means ± standard error. Data was evaluated using the 2way ANOVA test and significant differences compared with DMSO treatment group, respectively, are denoted by *(P ≤0.05).

Article Snippet: Monkey epithelial kidney cells Vero E6 (ATCC ® CRL-1586TM) were grown at 37°C and cultured in Eagle’s Minimum Essential Medium (EMEM, ATCC ® ) supplemented with 10% FBS and 100 U/ml penicillin-streptomycin.

Techniques: Inhibition, Infection, Staining, Sequencing, Binding Assay, Control, Western Blot